![]() Plant Cell 4:1371–1382įillatti JJ, Kiser J, Rose R, Comai L (1987) Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Nucleic Acids Res 12:387–395ĭietrich RA, Radke SE, Harada JJ (1992) Downstream DNA sequences are required to activate a gene expressed in the root cortex of embryos and seedlings. EMBO J 11:2345–2355ĭevereux J, Häberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Proc Natl Acad Sci USA 81:1991–1996ĭenecke J, De Rycke R, Botterman J (1992) Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope. M Dekker, New York, pp 103–128Ĭhurch GM, Gilbert W (1984) Genomic sequencing. ![]() In: Waisel Y, Eshel A, Kafkafi U (eds) Plant roots: The hidden half. Bot Gaz 138:71–79Ĭharlton WA (1991) Lateral root initiation. by means of colchicine inhibition of cell division. Plant Physiol 87:414–419Ĭharlton WA (1977) Evaluation of sequence and rate of lateralroot initiation in Pontederia cordata L. Bot Gaz 143:341–352īlakely LM, Blakely RM, Colowit PM, Elliott DS (1988) Experimental studies on lateral root formation in radish seedling roots. General methods, developmental stages and spontaneous formation of laterals. Plant Physiol 50:35–42īlakely LM, Durham M, Evans TA, Blakely RM (1982) Experimental studies on lateral root formation in radish seedling roots. Mol Gen Genet 203:8–14īlakely LM, Rodaway SJ, Hollen LB, Croker SG (1972) Control and kinetics of branch root formation in cultured root segments of Haplopappus ravenii. Plant Cell 2:225–233īarlow PW, Adam JS (1988) The position and growth of lateral roots on cultured root axes of tomato, Lycopersicon esculentum (Solanaceae) Plant Syst Evol 158:141–154īernatzky R, Tanksley SD (1986) Majority of random cDNA clones correspond to single loci in the tomato genome. Plant Physiol 89:743–752Īn G, Costa MA, Ha S-B (1990) Nopaline synthase promoter is wound inducible and auxin inducible. ![]() The putative mature protein exhibits 79% amino acid identity to a protein encoded by a gene ( GAST1) that is induced by gibberellic acid in tomato shoots.Īlliotte T, Tiré C, Engler G, Peleman J, Caplan A, Van Montagu M, Inzé D (1989) An auxin-regulated gene of Arabidopsis thaliana encodes a DNA-binding protein. The predicted RSI-1 protein is rich in cysteine, lysine and proline, and includes an N-terminal region with characteristics of a signal peptide. In roots from seedlings with high activity, GUS expression was also observed in the root cap and vascular tissue. GUS activity was observed in both lateral and adventitious root initials, including very early initials, and persisted until shortly after the lateral emerged from the parent tissue. Approximately 1 kb of the 5′ flanking DNA was linked to the β-glucuronidase (GUS) protein coding region and tested for expression in transgenic tomato seedlings. A gene ( RSI-1) corresponding to the TR132 cDNA was cloned and characterized with regard to its nucleotide sequence, transcription start site and chromosomal map position. A cDNA clone, TR132, was identified that hybridized to a transcript that was induced within 4 h of auxin treatment and increased tenfold by 72 h. To identify genes that are activated during lateral root initiation, a cDNA library was made with RNA from roots treated with auxin and differentially screened with radioactive probes made from RNA isolated from treated and untreated roots. VFN8) seedling roots is increased over eightfold in response to 1.6 μM α-naphthaleneacetic acid (NAA). The frequency of lateral root initiation in tomato ( Lycopersicon esculentum Mill cv.
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